Parallel Proteomic Comparison of Mutants With Altered Carbon Metabolism Reveals Hik8 Regulation of PII Phosphorylation and Glycogen Accumulation in a Cyanobacterium
Chengcheng Huang, Xiaoxiao Duan, Haitao Ge, Zhen Xiao, Limin Zheng, Gaojie Wang, Jinghui Dong, Yan Wang, Yuanya Zhang, Xiahe Huang, Hongyu An, Wu Xu, Yingchun Wang
Abstract
Comparative proteomics was simultaneously performed for five knockout mutants of Synechocystis each depleted of an important regulatory protein for carbon metabolism (RCM). Proteins uniquely or commonly regulated by the RCM proteins were identified. Particularly, dramatic hyperphosphorylation of P II on S49 was also identified uniquely in the hik8-knockout mutant that is concomitant with significantly reduced glycogen content. Further experimental evidence suggests that Hik8 regulates glycogen accumulation through negative regulation of P II phosphorylation.
Topics & Concepts
PhosphorylationBiologyMutantCell biologySignal transductionBiochemistrySerineResponse regulatorProteomeGenePhotosynthetic Processes and MechanismsAlgal biology and biofuel productionMicrobial Community Ecology and Physiology