Minimal Pathway for the Regeneration of Redox Cofactors
Michele Partipilo, Eleanor Ewins, Jacopo Frallicciardi, Tom Robinson, Bert Poolman, Dirk Jan Slotboom
Abstract
available again for the first reaction. The pathway is functional in liposomes ranging from a few hundred nanometers in diameter (large unilamellar vesicles) up to several tens of micrometers (giant unilamellar vesicles) and remains active over a period of 7 days. We demonstrate that the downstream biochemical process of reduction of glutathione disulfide can be driven by the transfer of reducing equivalents from formate via NAD(P)H, thereby providing a versatile set of electron donors for reductive metabolism.
Topics & Concepts
FormateFormate dehydrogenaseNAD+ kinaseCofactorChemistryLiposomeRedoxVesicleBiochemistryNicotinamideReducing agentEnzymeBiophysicsMembraneBiologyOrganic chemistryCatalysisATP Synthase and ATPases ResearchMitochondrial Function and PathologyCancer, Hypoxia, and Metabolism