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N-acetyl-l-cysteine averts ferroptosis by fostering glutathione peroxidase 4

Jiashuo Zheng, Weijia Zhang, Junya Ito, Bernhard Henkelmann, Chenxi Xu, Eikan Mishima, Marcus Conrad

2025Cell chemical biology37 citationsDOIOpen Access PDF

Abstract

, glutathione biosynthesis, or ferroptosis suppressor protein 1-is necessary for NAC and d-NAC to prevent ferroptosis. Additionally, we identify a broad range of reducing substrates for GPX4 in vitro, including β-mercaptoethanol. These findings provide new insights into the mechanisms underlying the protective effects of NAC and other potential GPX4-reducing substrates against ferroptosis.

Topics & Concepts

CysteineChemistryGlutathionePeroxidaseBiochemistryEnzymeFerroptosis and cancer prognosisEpigenetics and DNA MethylationMicroRNA in disease regulation