MXene Bimetallic Coating Synergistic Enhanced Colorimetric-Raman Dual Signal-Based Immunochromatographic Assay for Advancing Detection Performance
Youwen Deng, Zhiheng Gao, Ziqiong Lin, Zehao Yang, Meng‐Fang Lin, Zhenlin Xu, Hongtao Lei, Xiangmei Li
Abstract
Herein, a three-dimensional thin film-like multifunctional MXene bimetallic coating material (Ti 3 C 2 @Au–Ag) with strong color intensity, high surface-enhanced Raman scattering (SERS) activity, and strong antibody affinity (1.00 × 10 8 M –1 ) was prepared. It was the first time that Ti 3 C 2 @Au–Ag-based colorimetric-SERS dual-signal immunochromatographic assay (ICA) was developed for the detection of dexamethasone, achieving the limits of detection of 0.0089, 0.14, and 0.084 μg/kg for milk, beef, and pork in colorimetric mode and 0.0015, 0.060, and 0.075 μg/kg in SERS mode. It was up to 200-fold more sensitive than the reported ICAs. The recoveries were 82.0%–112.6%, and the coefficients of variation were 1.4%–13.7%. The Ti 3 C 2 @Au–Ag-ICA was verified by LC–MS/MS in the application on 30 real samples with a correlation coefficient greater than 0.98. This study can provide efficient theoretical and practical value for the development of a colorimetric-SERS dual-signal immunoassay platform.