Open-top multisample dual-view light-sheet microscope for live imaging of large multicellular systems
Franziska Moos, Simon Suppinger, Gustavo de Medeiros, Koen C. Oost, Andrea Boni, Camille Rémy, Sera Lotte Weevers, Charisios D. Tsiairis, Petr Strnad, Prisca Liberali
Abstract
Multicellular systems grow over the course of weeks from single cells to tissues or even full organisms, making live imaging challenging. To bridge spatiotemporal scales, we present an open-top dual-view and dual-illumination light-sheet microscope dedicated to live imaging of large specimens at single-cell resolution. The configuration of objectives together with a customizable multiwell mounting system combines dual view with high-throughput multiposition imaging. We use this microscope to image a wide variety of samples and highlight its capabilities to gain quantitative single-cell information in large specimens such as mature intestinal organoids and gastruloids.