Oxford Nanopore R10.4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or reference polishing
Mantas Sereika, Rasmus Hansen Kirkegaard, Søren Michael Karst, Thomas Yssing Michaelsen, Emil A. Sørensen, Rasmus Dam Wollenberg, Mads Albertsen
Abstract
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-finished microbial genomes from isolates or metagenomes without short-read or reference polishing.
Topics & Concepts
Nanopore sequencingNanoporeGenomePolishingBacterial genome sizeBiologyMetagenomicsComputational biologyDNA sequencingGeneNanotechnologyGeneticsMaterials scienceComposite materialGenomics and Phylogenetic StudiesMicrobial Community Ecology and PhysiologyBacteriophages and microbial interactions