L-arginine modified mesoporous bioactive glass with ROS scavenging and NO release for periodontitis treatment
Haiyan Yao, Emine Sumeyra Turali Emre, Yuan Fan, Jiaolong Wang, Feng Liu, Junchao Wei
Abstract
Periodontitis is a chronic inflammatory disease characterized by progressive alveolar bone resorption, and excessive reactive oxygen species (ROS) is a key factor to disease progression. Therefore, scavenging ROS to alleviate inflammation and promote bone regeneration are promising strategies to treat periodontitis. In this study, L-arginine (L-Arg) was used to modify mesoporous bioactive glass (MBG), forming L-Arg modified MBG (MBG@L-Arg), which showed effective ROS-scavenging and NO release properties in cells, and realize the protection and restoration of cell's activity in ROS-rich microenvironment. Furthermore, MBG@L-Arg can induce macrophage polarization from M1 to M2 phenotype, and promote the osteogenic differentiation of MC3T3-E1 cells and human periodontal ligament stem cells (hPDLSCs). MBG@L-Arg also regulated anti-inflammatory and antioxidant systems by inhibiting the NF- κ B signaling pathway and activating the Nrf2 signaling pathway. Besides, NO-PKG signaling pathway was also activated, further promoting bone regeneration. The in vivo results demonstrated that MBG@L-Arg can efficiently inhibit inflammation-induced tissue destruction and promote osteogenesis regeneration. The quantitative bone loss in MBG@L-Arg group was 1.03 ± 0.05 mm, significantly lower than that of the periodontitis group (1.47 ± 0.13 mm), implying that MBG@L-Arg can work as multifunctional materials for periodontal tissue regeneration. • A new hybrid MBG@L-Arg with the properties of ROS scavenging and NO release was prepared. • MBG@L-Arg can regulate the NF- κ B, Nrf2 and NO-PKG signaling pathways, and show strong anti-inflammatory and osteogenic activities. • MBG@L-Arg can efficiently treat periodontitis, and promote the regeneration of alveolar bone.