Identification of an Intermediate Form of Ferredoxin That Binds Only Iron Suggests That Conversion to Holo-Ferredoxin Is Independent of the ISC System in Escherichia coli
Xiaojun Ren, Feng Liang, Zhengfen He, Bingqian Fan, Zhirong Zhang, Xiudi Guo, Yukuan Du, Yilin Pang, Jianghui Li, Jianxin Lyu, Guoqiang Tan
Abstract
cells. The possibility remains that Fdx can effectively accept clusters from multiple sources. Nevertheless, our results suggest that Fdx has a strong iron binding activity that contributes to the assembly of its own [2Fe-2S] cluster and that Fdx acts as a temperature sensor to regulate Isc system-mediated iron-sulfur cluster biogenesis.
Topics & Concepts
FerredoxinOperonBiogenesisEscherichia coliChemistryCrystallographyStereochemistryBiochemistryEnzymeGeneMetalloenzymes and iron-sulfur proteinsTrace Elements in HealthMetal-Catalyzed Oxygenation Mechanisms