Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes
Jina Ko, Martin Wilkovitsch, Juhyun Oh, Rainer H. Köhler, Evangelia Bolli, Mikaël J. Pittet, Claudio Vinegoni, David B. Sykes, Hannes Mikula, Ralph Weissleder, Jonathan Carlson
Topics & Concepts
Bioorthogonal chemistryImmunofluorescenceFluorescenceFluorescent labellingCyclingChemistryCell biologyBiophysicsBiologyAntibodyImmunologyOpticsPhysicsHistoryCombinatorial chemistryArchaeologyClick chemistryAdvanced Biosensing Techniques and ApplicationsAdvanced biosensing and bioanalysis techniquesAdvanced Fluorescence Microscopy Techniques