Co-Localization of Resistance and Metabolic Quantitative Trait Loci on Carrot Genome Reveals Fungitoxic Terpenes and Related Candidate Genes Associated with the Resistance to Alternaria dauci
Claude Koutouan, V. Le Clerc, Anita Suel, Latifa Hamama, Patricia Claudel, David Halter, Raymonde Baltenweck, Philippe Hugueney, Jean‐François Chich, Sitti Anlati Moussa, Clémentine Champlain, Sébastien Huet, Linda Voisine, Sandra Pelletier, Sandrine Balzergue, Wilfried Chevalier, Emmanuel Geoffriau, Mathilde Briard
Abstract
, is the most damaging foliar disease of carrot. Some carrot genotypes exhibit partial resistance to this pathogen and resistance Quantitative Trait Loci (rQTL) have been identified. Co-localization of metabolic QTL and rQTL identified camphene, α-pinene, α-bisabolene, β-cubebene, caryophyllene, germacrene D and α-humulene as terpenes potentially involved in carrot resistance against ALB. By combining genomic and transcriptomic analyses, we identified, under the co-localization regions, terpene-related genes which are differentially expressed between a resistant and a susceptible carrot genotype. These genes include five terpene synthases and twenty transcription factors. In addition, significant mycelial growth inhibition was observed in the presence of α-humulene and caryophyllene.