A GTP-synthesizing ribozyme selected by metabolic coupling to an RNA polymerase ribozyme
Arvin Akoopie, Joshua T. Arriola, Douglas Magde, Ulrich F. Müller
Abstract
emulsion droplets. Several functional RNAs were identified, one of which was characterized in more detail. Under optimized reaction conditions, this ribozyme produced GTP at a rate 18,000-fold higher than the uncatalyzed rate, with a turnover of 1.7-fold, and supported the incorporation of GTP into RNA oligomers in tandem with an RNA polymerase ribozyme. These results are discussed in the context of early life forms.
Topics & Concepts
RibozymeLigase ribozymeGuanosineVS ribozymeBiochemistryNucleoside triphosphateRNAGTP'NucleotideNucleosideHairpin ribozymeRNA polymerasePolymeraseChemistryGuanosine triphosphateBiologyEnzymeGeneRNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyDNA and Nucleic Acid Chemistry