Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy
Cheng‐Ying Chu, Yi‐Ching Lee, Cheng-Han Hsieh, Chi‐Tai Yeh, Tsu‐Yi Chao, Po‐Hung Chen, I‐Hsuan Lin, Tsung‐Han Hsieh, Jing-Wen Shih, Chia‐Hsiung Cheng, Che‐Chang Chang, Ping-Sheng Lin, Yuan‐Li Huang, Tsung-Ming Chen, Yun Yen, David K. Ann, Hsing‐Jien Kung
Abstract
We uncovered TREM1/CCL2 activation, in addition to restored ASS1 expression, as a key pathway involved in full ADI-resistance in breast and prostate cancer models.
Topics & Concepts
CRISPRCas9Computational biologyKnockout mouseArginineBiologyMedicineGeneticsGeneAmino acidCancer Research and TreatmentsAnesthesia and Neurotoxicity ResearchVirus-based gene therapy research