Successive High-Resolution (H<sub>2</sub>O)<sub><i>n</i></sub>-GCIB and C<sub>60</sub>-SIMS Imaging Integrates Multi-Omics in Different Cell Types in Breast Cancer Tissue
Hua Tian, Louis J. Sparvero, Tamil S. Anthonymuthu, Wan‐Yang Sun, Andrew A. Amoscato, Rong‐Rong He, Hülya Bayır, Valerian E. Kagan, Nicholas Winograd
Abstract
-SIMS (at 1.1 μm beam spot size). We revealed distinct variations of distribution and intensities of >150 key ions (e.g., lipids and important metabolites) in different types of the TME individual cells, such as actively proliferating tumor cells as well as infiltrating immune cells. The demonstrated feasibility of SIMS imaging to integrate the multi-omics profiling in the same tissue section at the single-cell level will lead to new insights into the role of lipid reprogramming and metabolic response in normal regulation or pathogenic discoordination of cell-cell interactions in a variety of tissue microenvironments.