Live attenuated coronavirus vaccines deficient in N7-Methyltransferase activity induce both humoral and cellular immune responses in mice
Zhen Zhang, Qianyun Liu, Yingming Sun, Jiali Li, Jiejie Liu, Ruangang Pan, Liu Cao, Xianying Chen, Yingjian Li, Yuzhen Zhang, Ke Xu, Deyin Guo, Li Zhou, Ke Lan, Yu Chen
Abstract
T cell responses, which can provide full protection against the challenge of a lethal-dose of MHV-A59. Collectively, this study provides an ideal strategy to design live attenuated vaccines for coronavirus by abolishing viral RNA N7-MTase activity. This approach may apply to other RNA viruses that encode their own conservative viral N7-methyltransferase.
Topics & Concepts
VirologyCoronavirusBiologyImmune systemMouse hepatitis virusAttenuated vaccineVirusCytotoxic T cellImmunologyGeneGeneticsCoronavirus disease 2019 (COVID-19)MedicineVirulenceIn vitroPathologyDiseaseInfectious disease (medical specialty)SARS-CoV-2 and COVID-19 ResearchViral gastroenteritis research and epidemiologyViral Infections and Immunology Research