Sepsis Disrupts Mitochondrial Function and Diaphragm Morphology
Thamires Siqueira Oliveira, Anderson Teixeira Santos, Cherley Borba Vieira de Andrade, Johnatas D. Silva, Natália Blanco, Nazareth N. Rocha, Juliana Woyames, Pedro Leme Silva, Patrícia R. M. Rocco, Wagner Seixas da‐Silva, Tânia Maria Ortiga-Carvalho, Flávia Fonseca Bloise
Abstract
BACKGROUND: The diaphragm is the primary muscle of inspiration, and its dysfunction is frequent during sepsis. However, the mechanisms associated with sepsis and diaphragm dysfunction are not well understood. In this study, we evaluated the morphophysiological changes of the mitochondrial diaphragm 5 days after sepsis induction. METHODS: expressions were evaluated by using qPCR. Diaphragm ultrastructure was assessed by electron microscopy, and mitochondrial physiology was investigated by high-resolution respirometry, Western blot, and qPCR. RESULTS: by 70%. CONCLUSION: The current model of sepsis induced diaphragm morphological changes, increased mitochondrial damage, and induced functional impairment. Thus, diaphragm damage during sepsis seems to be associated with mitochondrial dysfunction.