Protocol to study electrophysiological properties of hPSC-derived 3D cardiac organoids using MEA and sharp electrode techniques
Ravichandra Venkateshappa, Zehra Yildirim, Shane Rui Zhao, Matthew A. Wu, Francesca Vacante, Oscar J. Abilez, Joe Z. Zhang
Abstract
Continuing advancements in human pluripotent stem cell (hPSC)-derived complex three-dimensional (3D) cardiac tissues require the development of novel technologies or adaptation of existing technologies to understand the physiology of the derived 3D cardiac tissues. In this protocol, we describe the use of multielectrode array (MEA) and sharp electrode electrophysiology techniques to investigate the electrical properties of 3D cardiac organoids. This protocol deciphers the electrical behavior of 3D cardiac organoids at both the single-cell level and tissue level.
Topics & Concepts
OrganoidElectrophysiologyElectrodeProtocol (science)ChemistryMicroelectrodeNeuroscienceMedicineBiologyPathologyPhysical chemistryAlternative medicineCardiac electrophysiology and arrhythmiasNeuroscience and Neural EngineeringElectrostatic Discharge in Electronics