An amperometric cholesterol biosensor based on immobilization of cholesterol oxidase onto titanium dioxide nanoparticles
Bhawna Batra, Vinay Narwal, Sumit Sumit, Jyoti Ahlawat, Minakshi Sharma
Abstract
Cholesterol is an important amphipathic molecule and essential structural constituent of all animal cells. It is made up of a hydrocarbon chain, four hydrocarbon ring structure and a hydroxyl group. It is also used in Vitamin D biosynthesis. Cholesterol is of two types: LDL (low-density lipoproteins, bad cholesterol) and HDL (high-density lipoproteins, good cholesterol. High cholesterol increase risk of coronary heart disease. Proposed work describes a unique approach of immobilizing cholesterol oxidase onto TiO2NPs, modified Pencil Graphite electrode and its application in construction of an amperometric biosensor for determination of serum cholesterol.
Topics & Concepts
Cholesterol oxidaseCholesterolChemistryAmperometryBiosensorTitanium dioxideAmphiphileBiochemistryElectrodeElectrochemistryOrganic chemistryMaterials sciencePolymerCopolymerMetallurgyPhysical chemistryElectrochemical sensors and biosensorsElectrochemical Analysis and ApplicationsAnalytical Chemistry and Sensors