Anti-proliferative and cytotoxic effect of Iranian snake (Vipera raddei kurdistanica) venom on human breast cancer cells via reactive oxygen species-mediated apoptosis
Cyrus Jalili, Ehsan Malekara, Mona Pazhouhi, Iraj Rashidi
Abstract
BACKGROUND AND PURPOSE: ) venom on breast cancer cells were investigated. EXPERIMENTAL APPROACH: The effect of increasing concentrations of snake venom on breast cell viability was assessed by trypan blue, MTT, and lactate dehydrogenase measurements. Apoptosis was detected and quantified by fluorescent staining and DNA fragmentation assay. The expression level of some apoptotic-related genes was investigated using real-time polymerase chain reaction (RT-PCR). The Western blotting method was also used to detect the protein expression profiles in the cells. FINDINGS / RESULTS: < 0.05). The RT-PCR and western blot data confirmed the increase of apoptosis in cells treated with venom. CONCLUSION AND IMPLICATIONS: activation of apoptosis in breast cancer cells.