Optical Oxygen Sensing and Clark Electrode: Face-to-Face in a Biosensor Case Study
P. V. Melnikov, A. Yu. Alexandrovskaya, Alina O. Naumova, Vyacheslav A. Arlyapov, Olga A. Kamanina, Nadezhda Popova, Н. К. Зайцев, N. A. Yashtulov
Abstract
In the last decade, there has been continuous competition between two methods for detecting the concentration of dissolved oxygen: amerometric (Clark electrode) and optical (quenching of the phosphorescence of the porphyrin metal complex). Each of them has obvious advantages and disadvantages. This competition is especially acute in the development of biosensors, however, an unbiased comparison is extremely difficult to achieve, since only a single detection method is used in each particular study. In this work, a microfluidic system with synchronous detection of the oxygen concentration by two methods was created for the purpose of direct comparison. The receptor element is represented by Saccharomyces cerevisiae yeast cells adsorbed on a composite material, previously developed by our scientific group. To our knowledge, this is the first work of this kind in which the comparison of the oxygen detection methods is carried out directly.