Universal and high-fidelity DNA single nucleotide polymorphism detection based on a CRISPR/Cas12a biochip
Yong Chen, Yixin Mei, Xingyu Jiang
Abstract
(G636A, rs4986893) genes related to multiple cancers from 17 clinical blood samples. This CRISPR/Cas12a-based SNP genotyping method, being universal, accurate, and sensitive, will have broad applications in molecular diagnostics and clinical research.
Topics & Concepts
CRISPRBiochipGeneticsComputational biologyDNAFidelityBiologySingle-nucleotide polymorphismNucleotideComputer scienceGeneGenotypeTelecommunicationsCRISPR and Genetic EngineeringEvolution and Genetic DynamicsRNA and protein synthesis mechanisms