Spot Assay and Colony Forming Unit (CFU) Analyses–based sensitivity test for <em>Candida albicans</em> and <em>Saccharomyces cerevisiae</em>
Satya Ranjan Sahu, Bhabasha Gyanadeep Utkalaja, Shraddheya Kumar Patel, Narottam Acahrya
Abstract
of diverged genetic backgrounds subjected to several genotoxic chemicals simultaneously. We demonstrate it by testing the sensitivity of DNA polymerase defective yeast mutants by using spot analysis combined with colony forming unit (CFU) efficiency estimation. The method is very simple and inexpensive, does not require any sophisticated equipment, can be completed in 2-3 days, and provides both qualitative and quantitative data. We also recommend the use of this reliable methodology for assaying the sensitivity of these and other fungal species to antifungal drugs and xenobiotic factors.
Topics & Concepts
Candida albicansSaccharomyces cerevisiaeBiologySensitivity (control systems)YeastReplication (statistics)DNA replicationDNAGenetic screenGeneMutantComputational biologyCell biologyGeneticsMicrobiologyElectronic engineeringVirologyEngineeringAntifungal resistance and susceptibilityFungal Infections and StudiesProbiotics and Fermented Foods