Detecting telomerase activity at the single-cell level using a CRISPR-Cas12a-based chip
Yateng Jiang, Yanping Wang, Wen Luo, Xiaowei Luan, Zhibin Zhang, Yongchun Pan, Bangshun He, Yanfeng Gao, Yujun Song
Abstract
-cleavage activity of CRISPR-Cas12a. This amplification-based assay could be integrated with a microfluidic chip to enable highly sensitive detection of telomerase activity at the single-cell level, offering promising advancements in early cancer diagnosis.
Topics & Concepts
CRISPRTelomeraseChipComputational biologyComputer scienceChemistryBiologyGeneticsGeneTelecommunicationsTelomeres, Telomerase, and SenescenceAdvanced biosensing and bioanalysis techniquesCRISPR and Genetic Engineering