Constitutive cell surface expression of ZZ domain for the easy preparation of yeast-based immunosorbents
Kohei Katsurada, Masahiro Tominaga, Misato Kaishima, Hiroko Kato, Toshihide Matsuno, Chiaki Ogino, Akihiko Kondo, Jun Ishii, Katsumi Takayama
Abstract
We describe a novel expression cassette that enables efficient and constitutive expression of the ZZ domain derived from Staphylococcus aureus protein A on the yeast cell surface to easily prepare yeast-based immunosorbents. Using this expression cassette containing the PGK1 promoter, a secretion signal derived from α-factor, and a Flo1-derived anchor protein, we successfully created a yeast-based immunosorbent for human serum albumin.
Topics & Concepts
YeastSurface proteinSaccharomyces cerevisiaeSecretionCellChemistryBiologyCell biologyMicrobiologyBiochemistryVirologyMonoclonal and Polyclonal Antibodies ResearchCellular transport and secretionBiochemical and Structural Characterization