Inhibitory Effect of Epigallocatechin Gallate-Silver Nanoparticles and Their Lysozyme Bioconjugates on Biofilm Formation and Cytotoxicity
Brahmaiah Meesaragandla, Shahar Hayet, Tamir Fine, Una Janke, Liraz Chai, Mihaela Delcea
Abstract
biofilm formation at concentrations larger than 40 nM, and EGCG-AgNP-lysozyme bioconjugates inhibited biofilms at concentrations above 80 nM. Cytotoxicity assays performed with human cells showed a reverse trend, where EGCG-AgNPs barely affected human cell viability while EGCG-AgNP-lysozyme bioconjugates severely hampered viability. Our results therefore demonstrate that EGCG-AgNPs may be used as noncytotoxic antibiofilm agents.
Topics & Concepts
LysozymeBiofilmChemistryCytotoxicitySilver nanoparticleEpigallocatechin gallateBacillus subtilisMicrobiologyViability assayCircular dichroismAntibacterial activityIn vitroBacteriaBiochemistryNanoparticleNanotechnologyBiologyPolyphenolAntioxidantMaterials scienceGeneticsNanoparticles: synthesis and applicationsAdvanced Nanomaterials in CatalysisBacterial biofilms and quorum sensing