A genetically encoded fluorescent biosensor for extracellular l-lactate
Yusuke Nasu, Ciaran Murphy‐Royal, Yurong Wen, Jordan N. Haidey, Rosana S. Molina, Abhi Aggarwal, Shuce Zhang, Yuki Kamijo, Marie-Ève Paquet, Kaspar Podgorski, Mikhail Drobizhev, Jaideep S. Bains, M. Joanne Lemieux, Grant R. Gordon, Robert E. Campbell
Abstract
L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.
Topics & Concepts
ExtracellularBiosensorIntracellularFluorescenceCell biologyBiochemistryGreen fluorescent proteinChemistryBiologyBiophysicsGeneQuantum mechanicsPhysicsNeuroscience and Neuropharmacology ResearchReceptor Mechanisms and Signalingbioluminescence and chemiluminescence research