Paper-based electrochemical device for early detection of integrin αvβ6 expressing tumors
Stefano Cinti, Stefano Tomassi, Chiara Ciardiello, R. Migliorino, Marinella Pirozzi, Alessandra Leone, Elena Di Gennaro, Virginia Campani, Giuseppe De Rosa, Vincenzo Maria D’Amore, Salvatore Di Maro, Greta Donati, Sima Singh, Ada Raucci, Francesco Saverio Di Leva, Horst Kessler, Alfredo Budillon, Luciana Marinelli
Abstract
Abstract Despite progress in the prevention and diagnosis of cancer, current technologies for tumor detection present several limitations including invasiveness, toxicity, inaccuracy, lengthy testing duration and high cost. Therefore, innovative diagnostic techniques that integrate knowledge from biology, oncology, medicinal and analytical chemistry are now quickly emerging in the attempt to address these issues. Following this approach, here we developed a paper-based electrochemical device for detecting cancer-derived Small Extracellular Vesicles (S-EVs) in fluids. S-EVs were obtained from cancer cell lines known to express, at a different level, the αvβ6 integrin receptor, a well-established hallmark of numerous epithelial cancer types. The resulting biosensor turned out to recognize αvβ6-containing S-EVs down to a limit of 0.7*10 3 S-EVs/mL with a linear range up to 10 5 S-EVs /mL, and a relative standard deviation of 11%, thus it may represent a novel opportunity for αvβ6 expressing cancers detection.