A Minimal Replicon Enables Efficacious, Species-Specific Gene Deletion in Chlamydia and Extension of Gene Knockout Studies to the Animal Model of Infection Using Chlamydia muridarum
Kenneth A. Fields, Maria D. Bodero, Kaylyn R. Scanlon, Travis J. Jewett, Katerina Wolf
Abstract
invasion and fitness assays. The virulence of the C. muridarum strains was then assessed in a murine infection model. Our approach represents a novel and efficient strategy for targeted genetic manipulation in Chlamydia beyond C. trachomatis L2. This advance will support comparative studies of species-specific infection biology and enable studies in a well-established murine model of chlamydial pathogenesis.
Topics & Concepts
BiologyChlamydia trachomatisChlamydiaPlasmidTissue tropismRepliconVirologyGeneticsMutagenesisMicrobiologyGeneVirulenceChlamydiaceaeTropismMutantVirusReproductive tract infections researchGut microbiota and healthPlant and fungal interactions