High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition
Sridhar Selvaraj, William N. Feist, Sébastien Viel, Sriram Vaidyanathan, Amanda M. Dudek, Marc Gastou, Sarah J. Rockwood, Freja K. Ekman, Aluya Oseghale, Liwen Xu, Mara Pavel-Dinu, Sofia E. Luna, M. Kyle Cromer, Ruhi Sayana, Natalia Gomez‐Ospina, Matthew H. Porteus
Topics & Concepts
IndelTransgeneDNA-PKcsBiologyGeneComputational biologyHuman genomeDNAHomology (biology)Homologous recombinationGeneticsDNA repairGenomeSingle-nucleotide polymorphismGenotypeCRISPR and Genetic EngineeringPluripotent Stem Cells ResearchRNA Interference and Gene Delivery