A primer to directed evolution: current methodologies and future directions
Lara Sellés Vidal, Mark Isalan, John Heap, Rodrigo Ledesma‐Amaro
Abstract
evolution experiments performed by Sol Spiegelman in 1967, a wide range of techniques have been developed to tackle the main two steps of directed evolution: genetic diversification (library generation), and isolation of the variants of interest. This review covers the main modern methodologies, discussing the advantages and drawbacks of each, and hence the considerations for designing directed evolution experiments. Furthermore, the most recent developments are discussed, showing how advances in the handling of ever larger library sizes are enabling new research questions to be tackled.
Topics & Concepts
Current (fluid)Primer (cosmetics)Computational biologyComputer scienceData scienceBiologyEngineeringElectrical engineeringPhysicsThermodynamicsMicrobial Metabolic Engineering and BioproductionEvolution and Genetic DynamicsRNA and protein synthesis mechanisms