Identification of Single Amino Acid Chiral and Positional Isomers Using an Electrostatically Asymmetric Nanopore
Jiajun Wang, Jigneshkumar Dahyabhai Prajapati, Fan Gao, Yi‐Lun Ying, Ulrich Kleinekathöfer, Mathias Winterhalter, Yi‐Tao Long
Abstract
mutant have been discriminated. Both chiral isomers were not applicable to be distinguished by mass spectroscopy approaches. These findings highlight a novel sensing mechanism for identifying single amino acids in single peptides and even for achieving single-molecule protein sequencing.
Topics & Concepts
ChemistryPeptideProtonationAmino acidChirality (physics)MembraneStereochemistryNanoporeBiophysicsCrystallographyBiochemistryNanotechnologyIonOrganic chemistryChiral symmetry breakingMaterials scienceQuarkBiologyPhysicsQuantum mechanicsNambu–Jona-Lasinio modelNanopore and Nanochannel Transport StudiesRNA and protein synthesis mechanismsAdvanced biosensing and bioanalysis techniques