Rutin present in <i>Alibertia edulis</i> extract acts on human platelet aggregation through inhibition of cyclooxygenase/thromboxane
Caroline Honaiser Lescano, Fernando Freitas de Lima, Cláudia Andréa Lima Cardoso, Silvia Cristina Heredia Vieira, Fabíola Z. Mónica, Ivan Pires de Oliveira
Abstract
, phosphorylation of vasodilator-stimulated phosphoprotein Ser157 and Ser239, and protein kinase Cβ, which were significantly reduced by Alibertia edulis leaf extract, rutin, and caffeic acid as well mixtures of rutin/caffeic acid. Cyclooxygenase 1 activity was inhibited for Alibertia edulis leaf extract, rutin and caffeic acid. These inhibitions were firsrtly explored by specific stabilization of rutin and caffeic acid compared to diclofenac at the catalytic site from docking score and free-energy dissociation profiles. Then, simulations detailed the rutin interactions close to the heme group and Tyr385, responsible for catalyzing the conversion of arachidonic acid to its products. Our results reveal the antiplatelet aggregation properties of Alibertia edulis leaf extract, rutin and caffeic acid providing pharmacological information about its origin from cyclooxygenase 1 inhibition and its downstream pathway.