Microfluidic harvesting of breast cancer tumor spheroid-derived extracellular vesicles from immobilized microgels for single-vesicle analysis
Xilal Y. Rima, Jingjing Zhang, Luong T. H. Nguyen, Aaron Rajasuriyar, Min Jin Yoon, Chi‐Ling Chiang, Nicole Walters, Kwang Joo Kwak, L James Lee, Eduardo Reátegui
Abstract
μBRs were capable of recapitulating heterogeneity at a cellular and vesicular degree, indicating the utility and robustness of the microfluidic system to investigate physiologically relevant EVs in breast cancer and other disease models.
Topics & Concepts
SpheroidMicrofluidicsChemistryBreast cancerMicrovesiclesVesicleBiophysicsTumor microenvironmentExtracellular vesicleCancer cellCell biologyNanotechnologyCancerMaterials scienceCancer researchBiologyIn vitroTumor cellsBiochemistryMembranemicroRNAGeneGeneticsExtracellular vesicles in disease3D Printing in Biomedical ResearchCancer Cells and Metastasis