Clinical hematological and biochemical parameters in Swiss, BALB/c, C57BL/6 and B6D2F1 <i>Mus musculus</i>
Giorgio Silva‐Santana, Juliet Cunha Bax, Débora Cristina Silva Fernandes, Daniela Tendler Leibel Bacellar, Cleber Hooper, Alexandre Alves Souza Oliveira Dias, Cristina Barbosa da Silva, Aline Moreira de Souza, Simone G. Ramos, Ricardo Alexandre dos Santos, Thainara Ramos Pinto, Mariana Antunes Ramão, Ana Luíza Mattos‐Guaraldi
Abstract
Abstract Background Animal models are widely used in scientific research in order to obtain information from a whole organism under a specific set of experimental conditions. Various lineages of mice have been used to investigate diseases and new therapeutic strategies, and, consequently, hematological and biochemical tests in these laboratory animals are essential to validate scientific studies. Our study seeks to establish reference values for hematological and biochemical parameters of four lineages of mice. Methods We evaluated the hematological and biochemical profiles of 20 males and 20 females from the lineages Swiss (heterogeneous), BALB/c and C57BL/6 (isogenic), and B6D2F1 (hybrid), totaling 160 mice. Analysis were standardized using the systems pocH‐100iV Diff ™ for 19 hematological parameters and VITROS ® 350 for 12 biochemical parameters. Results Results are shown as means and standard deviation, grouped by lineage and genre. Comparing the values obtained in this study with the values from previous studies, some variations were detected, which could be explained by differences in methodologies or individual variability. Conclusion Thus our study shows that knowledge and disclosure of the values of physiological parameters of laboratory animals is necessary, and emphasises the importance of considering variations influenced by gender, lineage and genotype in the choice of the best experimental model.