Prime editing-mediated precise knockin of protein tag sequences in the rice genome

Abstract

Accurately labeling proteins in living plant cells has long been a challenge and can be addressed by targeted insertion of tag sequences in a given locus. Recent optimized plant prime editors (PEs) enable efficient programmable installation of small insertions or deletions, including insertions of short sequences (Li et al., 2022a, 2022b; Jiang et al., 2022; Xu et al., 2022; Zong et al., 2022; Zou et al., 2022). To investigate whether prime editing can be used to tag endogenous proteins in rice, we made use of the enpPE2 system described in our previous report (Li et al., 2022b).

Topics & Concepts