Micro-Raman spectroscopic analysis of liquid–liquid phase separation
Suin Choi, So Yeon Chun, Kyungwon Kwak, Minhaeng Cho
Abstract
O) inside the droplets exhibited a distinct Raman spectrum from that of the bulk water, suggesting that the time-dependent change in the hydration environment in the protein droplets during the process of LLPS can be studied. These results demonstrate that the superior spatial resolution of micro-Raman spectroscopy offers significant advantages in investigating the molecular mechanisms of LLPS and following LSPT processes.
Topics & Concepts
Raman spectroscopyChemistryChemical physicsProtein aggregationMicroscopyBiophysicsPhase (matter)Analytical Chemistry (journal)Chemical engineeringChromatographyOrganic chemistryBiochemistryOpticsPhysicsEngineeringBiologyRNA Research and SplicingProtein Structure and DynamicsHeat shock proteins research