Efficient regio- and stereo-selective C–H bond hydroxylation of steroids using an engineered heme-thiolate peroxygenase biocatalyst
Jinia Akter, Eva F. Hayball, Stephen G. Bell
Abstract
A steroid hydroxylating monooxygenase enzyme was converted into a peroxygenase to enable the stereoselective hydroxylation of C–H bonds in these complex molecules simply by adding hydrogen peroxide.
Topics & Concepts
HydroxylationChemistryBiocatalysisMonooxygenaseHydrogen peroxideStereoselectivityHemeCatalysisHydrogen bondStereochemistryCombinatorial chemistryMoleculeEnzymeCytochrome P450Organic chemistryReaction mechanismPharmacogenetics and Drug MetabolismMetal-Catalyzed Oxygenation MechanismsSynthesis and Catalytic Reactions