G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p
Jun Gao, Zhaofeng Gao, Andrea Putnam, Alicia K. Byrd, Sarah Venus, John C. Marecki, A. Edwards, H Löwe, Eckhard Jankowsky, Kevin D. Raney
Abstract
G-quadruplex DNA interacts with the N-terminal intrinsically disordered domain of the DEAD-box helicase Ded1p, diminishing RNA unwinding activity but enhancing liquid-liquid phase separation of Ded1p in vitro and in cells. The data highlight multifaceted effects of quadruplex DNA on an enzyme with intrinsically disordered domains.
Topics & Concepts
HelicaseRNA Helicase APrimaseDNADEAD boxBiophysicsChemistryG-quadruplexRNABiologyBiochemistryReverse transcriptaseGeneDNA and Nucleic Acid ChemistryRNA and protein synthesis mechanismsRNA Research and Splicing