Quantification of Amine- and Alcohol-Containing Metabolites in Saline Samples Using Pre-extraction Benzoyl Chloride Derivatization and Ultrahigh Performance Liquid Chromatography Tandem Mass Spectrometry (UHPLC MS/MS)
Brittany Widner, Melissa C. Kido Soule, Frank Ferrer-González, Mary Ann Moran, Elizabeth B. Kujawinski
Abstract
-labeled benzoyl chloride. We optimized derivatization, extraction, and sample preparation for field and culture samples and evaluated matrix-derived biases. We have optimized this quantitative method for 73 common metabolites, of which 50 cannot be quantified without derivatization due to low extraction efficiencies. Of the 73 metabolites, 66 were identified in either culture media or seawater and 45 of those were quantified. This derivatization method is sensitive (detection limits = pM to nM), rapid (∼5 min per sample), and high throughput.
Topics & Concepts
DerivatizationChemistryChromatographyExtraction (chemistry)Solid phase extractionMass spectrometryBenzoyl chlorideSample preparationDansyl chlorideTandem mass spectrometryLiquid chromatography–mass spectrometryElectrospray ionizationElectrosprayOrganic chemistryMetabolomics and Mass Spectrometry StudiesIsotope Analysis in EcologyMicrobial Community Ecology and Physiology