Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA
Kai Wang, Ruei‐Zeng Lin, Xuechong Hong, Alex H. M. Ng, Chin Nien Lee, Joseph Neumeyer, Gang Wang, Xi Wang, Minglin Ma, William T. Pu, George M. Church, Juan M. Melero‐Martin
Abstract
was critical, and bypassing the mesodermal stage produced putative h-iECs with reduced expansion potential and inability to form functional vessels. Our protocol has broad applications and could reliably provide an unlimited number of h-iECs for vascular therapies.
Topics & Concepts
Induced pluripotent stem cellCell biologyStem cellHuman Induced Pluripotent Stem CellsEndothelial stem cellBiologyCellular differentiationEmbryonic stem cellGeneIn vitroGeneticsPluripotent Stem Cells Research3D Printing in Biomedical ResearchCRISPR and Genetic Engineering