Simple and Efficient One-Pot Extraction Method for Phospholipidomic Profiling of Total Oil and Lecithin by Phosphorus-31 Nuclear Magnetic Resonance Measurements
Mirja Kaizer Ahmmed, Senni Bunga, Ian Stewart, Hong Tian, Alan Carne, Alaa El‐Din A. Bekhit
Abstract
The present study reports an efficient method using ethanol and hexane for lipid extraction (ETHEX) that is simpler and faster than the FOLCH (methanol/chloroform) and PALC (ethanol/hexane, a multi-step and time-consuming method) methods for determination of the phospholipid (PL) and fatty acid contents, using hoki roe as a model system. Substantial differences were found with the PALC and ETHEX methods, resulting in higher total lipid (14.6 ± 0.35 and 14.3 ± 0.08%, respectively) and lecithin (4.95 ± 0.08 and 4.89 ± 0.35%, respectively) yields compared to the FOLCH method (total lipid, 12.9 ± 0.35%; lecithin, 3.15 ± 0.35%). Phospholipids (LDPG, CL, LPS, SM, PE, LPC, PI, and PC) were found to partition in the methanol aqueous layer with the FOLCH method. Better phosphorus-31 nuclear magnetic resonance resolution and detection of PL, including lyso-PL, was obtained using D2O. The best extraction and detection of PL was achieved with the novel ETHEX method using D2O.