Multicenter Evaluation of an MIC-Based Aztreonam and Ceftazidime-Avibactam Broth Disk Elution Test
Harley Harris, Lili Tao, Emily Jacobs, Yehudit Bergman, Ayomikun Adebayo, Tsigedera Tekle, Shawna Lewis, Ashley Dahlquist, Taylor C. Abbey, Eric Wenzler, Romney M. Humphries, Patricia J. Simner
Abstract
Due to limited therapeutic options, there is a clinical need to assess the in vitro activity of the combination of aztreonam (ATM) and ceftazidime-avibactam (CZA) to guide the therapeutic management of multidrug-resistant (MDR) Gram-negative organism infections. We set out to develop a practical MIC-based broth disk elution (BDE) method to determine the in vitro activity of the combination ATM-CZA using readily available supplies and compare it to reference broth microdilution (BMD).
Topics & Concepts
Ceftazidime/avibactamAztreonamBroth microdilutionCeftazidimeMicrobiologyAvibactamMinimum inhibitory concentrationMedicineBiologyChemistryChromatographyAntibioticsBacteriaPseudomonas aeruginosaGeneticsAntibiotic Resistance in BacteriaClostridium difficile and Clostridium perfringens researchAntibiotics Pharmacokinetics and Efficacy