DNA nanomachine for visual detection of structured RNA and double stranded DNA
Daria A. Gorbenko, Liubov A. Shkodenko, Maria S. Rubel, Александр В. Слита, Ekaterina Nikitina, E. A. Martens, Dmitry M. Kolpashchikov
Abstract
Visual detection of ssRNA and dsDNA amplicons was achieved at room temperature without the need for a probe-analyte annealing stage. This approach uses a DNA nanostructure equipped with two analyte-binding arms. Highly selective binding of the third arm leads to the formation of a G-quadruplex structure capable of changing the solution color.
Topics & Concepts
AnalyteDNAAmpliconChemistryRNANanotechnologyG-quadruplexNanostructureDNA sequencingAptamerBiophysicsComputational biologyCombinatorial chemistryMaterials scienceMolecular biologyBiochemistryBiologyPolymerase chain reactionChromatographyGeneAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionDNA and Nucleic Acid Chemistry