Limited-resource preparable chitosan magnetic particles for extracting amplification-ready nucleic acid from complex biofluids
Sayantan Tripathy, Ashish Chalana, Arunansu Talukdar, Puspalata Rajesh, Abhijit Saha, Goutam Pramanik, Souradyuti Ghosh
Abstract
(on beads) LAMP was also probed. The required optimization of the amplification methods has been discussed. Overall, the turnaround time for the magnetocapture combined with NAAT was 1.5-2 h and is thus expected to aid in rapid clinical decision making. With the ease of preparation, reproducibility, and compatibility with downstream NAATs, we anticipate that these magnetic particles would facilitate the expansion and decentralization of nucleic acid-based diagnosis for limited-resource settings.
Topics & Concepts
Nucleic acidNucleic Acid Amplification TestsChaotropic agentNanotechnologyNucleic acid detectionExtraction (chemistry)ChemistryMaterials scienceComputer scienceChromatographyBiologyBiochemistryVirologyChlamydia trachomatisBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniquesMicrofluidic and Bio-sensing Technologies