Comparison of Zinc Concentrations in the Broth of Commercial Automated Susceptibility Testing Devices (Vitek 2, MicroScan, BD Phoenix, and Sensititre)
Tomefa E. Asempa, Christian M Gill, Vindana Chibabhai, David P. Nicolau
Abstract
Growing data on the impact of extracellular zinc concentration on metallo-β-lactamase-mediated resistance has shed light on the importance of susceptibility testing media. However, there are no studies documenting the amount of zinc in commonly utilized automated susceptibility testing (AST) platforms. This study reveals supraphysiologic zinc concentrations as well as large zinc variability among AST platforms and highlights the challenges this raises in the development of zinc-limited media.
Topics & Concepts
ZincBroth microdilutionChemistryMicrobiologyChromatographyFood scienceBiologyMinimum inhibitory concentrationAntimicrobialOrganic chemistryBacterial Identification and Susceptibility TestingAntibiotic Resistance in BacteriaAntimicrobial Resistance in Staphylococcus