Construction of a magnetic covalent organic framework with synergistic affinity strategy for enhanced glycopeptide enrichment
Bin Luo, Guohao Li, Zhiyu Li, Jia He, Juan Zhou, Lijuan Wu, Fang Lan, Yao Wu
Abstract
), and a good size-exclusion effect (1 : 500, IgG digests/BSA, m/m). More excitingly, a total of 1921 unique intact glycopeptides assigned to 1154 glycoproteins were identified from rat liver tissue; this performance is superior to that of commercial products. Additionally, the nanocomposite was successfully applied to enrich intact glycopeptides of exosomes extracted from healthy individuals and renal failure patients, providing a novel concept for the design of materials using a synergistic affinity strategy for sample preparation in glycoproteomics.
Topics & Concepts
Covalent bondGlycopeptideCovalent organic frameworkNanocompositeBoronic acidLigand (biochemistry)GlycanCombinatorial chemistrySurface modificationMembraneChemistryMaterials scienceNanotechnologyOrganic chemistryGlycoproteinBiochemistryReceptorPhysical chemistryAntibioticsCovalent Organic Framework ApplicationsAdvanced biosensing and bioanalysis techniquesGlycosylation and Glycoproteins Research