An orthogonally activatable CRISPR-Cas13d nanoprodrug to reverse chemoresistance for enhanced chemo-photodynamic therapy
Zheng Liu, Zhiyuan Feng, Mohan Chen, Jiayin Zhan, Rong Wu, Yang Shi, Yunsheng Xue, Ran Liu, Jun‐Jie Zhu, Jingjing Zhang
Abstract
studies demonstrated that URL can promote Pt(iv) prodrug activation and ROS generation and massively induce on-target drug accumulation by Cas13d-mediated drug resistance attenuation, delivering an ultimate chemo-photodynamic therapeutic performance in efficiently eradicating primary tumors and preventing further liver metastasis. Collectively, our results suggest that URL expands the Cas13d-based genome editing toolbox into prodrug nanomedicine and accelerates the discovery of new orthogonal therapeutic approaches.
Topics & Concepts
CRISPRPhotodynamic therapyChemistryCancer researchMedicineBiochemistryGeneOrganic chemistryNanoplatforms for cancer theranosticsAdvanced biosensing and bioanalysis techniquesPhotodynamic Therapy Research Studies