APEX2 Proximity Proteomics Resolves Flagellum Subdomains and Identifies Flagellum Tip-Specific Proteins in Trypanosoma brucei
Daniel E. Vélez‐Ramírez, Michelle M. Shimogawa, Sunayan S. Ray, Andrew Lopez, Shima Rayatpisheh, Gerasimos Langousis, Marcus Gallagher-Jones, Samuel Dean, James A. Wohlschlegel, Kent L. Hill
Abstract
Sleeping sickness is a neglected tropical disease caused by the protozoan parasite Trypanosoma brucei . The disease disrupts the sleep-wake cycle, leading to coma and death if left untreated. T. brucei motility, transmission, and virulence depend on its flagellum (cilium), which consists of several different specialized subdomains.
Topics & Concepts
FlagellumTrypanosoma bruceiCiliumMotilityVirulenceBiologyMotile ciliumTransmission (telecommunications)Cell biologyAfrican trypanosomiasisTrypanosomiasisMicrobiologyVirologyGeneGeneticsEngineeringElectrical engineeringTrypanosoma species research and implicationsInsect symbiosis and bacterial influencesCellular transport and secretion