High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip
Federico Sala, Michele Castriotta, Petra Paiè, Andrea Farina, Sarah D’Annunzio, Alessio Zippo, Roberto Osellame, Francesca Bragheri, Andrea Bassi
Abstract
Single-cell analysis techniques are fundamental to study the heterogeneity of cellular populations, which is the basis to understand several biomedical mechanisms. Light-sheet fluorescence microscopy is a powerful technique for obtaining high-resolution imaging of individual cells, but the complexity of the setup and the sample mounting procedures limit its overall throughput. In our work, we present an optofluidic microscope-on-chip with integrated microlenses for light-sheet shaping and with a fluidic microchannel that allows the automatic and continuous delivery of samples of a few tens of microns in size. The device is used to perform dual-color fluorescence analysis and 3D reconstruction of xenograft-derived mouse breast cancer cells.