Anethole rich Clausena heptaphylla (Roxb.) Wight & Arn., essential oil pharmacology and genotoxic efficiencies
Mohan Lal, Twahira Begum, Roktim Gogoi, Neelav Sarma, Sunita Munda, Sudin Kumar Pandey, Joyashree Baruah, Raghu Tamang, Samarjit Saikia
Abstract
Abstract Anethole, a widely used industrial flavoring agent is majorly sourced from anise and star anise. The present study is aimed to the in-depth pharmacological analysis i.e. anti-diabetic, skin whitening, neurodegenerative disorder inhibitory activities of anethole-rich Clausena heptaphylla leaf essential oil (ARCHEO) (88.59%) as revealed by the Gas Chromatography/Mass Spectrometry (GC/MS) analysis and further confirmed by proton nuclear magnetic resonance 1 H-NMR as well as to compare with standard compound anethole . ARCHEO (ABTS EC 50 6.97 ± 0.004 µg/mL; Protease assay 4.51 ± 0.004 µg/mL) outperformed the standard compound anethole (ABTS EC 50 9.48 ± 0.048 µg/mL; Protease assay EC 50 22.64 ± 0.016 µg/mL) in antioxidant and anti-inflammatory experiments. ARCHEO was also shown to be more effective than the reference compound anethole in terms of anti-diabetic activity (EC 50 22.35 ± 0.121 µg/mL), tyrosinase inhibitory activity (EC 50 16.45 ± 0.012 µg/mL), and anti-cholinesterase activity (EC 50 22.32 ± 0.016 µg/mL). However, ARCHEO exhibited lower antimicrobial activity towards all the tested microbes compared to standard compound anethole and as for the MIC, ARCHEO was effective only towards Salmonella typhimurium (60 µg/mL) , Streptococcus mutans (20 µg/mL) , and Aspergillus fumigatus (75 µg/mL) . ARCHEO (11.11%) and anethole (12.33%) showed no genotoxic effect based on Allium cepa assay mitotic index value. Thus, ARCHEO could be a commercially viable and widely available cheaper source of anethole, which has buoyant demand in the field of food flavoring, fragrance, and pharmaceutical industries.