Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor
Alena Rudkouskaya, Nattawut Sinsuebphon, Marien Ochoa, Sez-Jade Chen, Joseph E. Mazurkiewicz, Xavier Intes, Margarida Barroso
Abstract
Overall, our methodology enables for the first time simultaneous non-invasive monitoring of intracellular drug delivery and metabolic response in preclinical studies.
Topics & Concepts
Förster resonance energy transferFluorescence-lifetime imaging microscopyIn vivoMolecular imagingBiophysicsAlexa FluorPreclinical imagingChemistryFluorescenceBiologyQuantum mechanicsBiotechnologyPhysicsNanoplatforms for cancer theranosticsCancer Research and TreatmentsAdvanced Fluorescence Microscopy Techniques